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At Novoprotein, we carefully design a unique purification strategy for each individual protein. This enables us to purify most untagged recombinant proteins within three-steps: capture, intermediate step, and polish. Our strategy ensures a high recovery rate. For tagged proteins, our two-step purification method can achieve greater than 95% purity in most cases.
Why choose us?
- Multiple purification systems in a temperature controlled environment
- Extensive collection of chromatography media
- Creative configurations of chromatography columns tailored to each protein
- Vigorous quality assurance programs to ensure the required quality and reproducibility
- Production capacity of up to 30 grams
- Greater than 98% purity
- Strong trouble shooting and difficult protein handling experience
See testimonials and case studies
For more information about our yeast expression service, contact us at services@novoprotein.com or visit our Service page. Want to know how other scientists speak of us? Check out our References.
Characteristic | E.coli | Yeast | Insect | Mammalian | |
---|---|---|---|---|---|
Proteolytic cleavage | usually not | yes | yes | yes | |
Proteolytic | N-linked | no | high mannose | simple | complex |
O-linked | no | yes | yes | yes | |
Secretion | to periplasm | to medium | to medium | to medium | |
Folding | usually not | improper | proper | proper | |
Phosphorylation | no | yes | yes | yes | |
Acetylation | no | yes | yes | yes | |
Amidation | no | yes | yes | yes | |
Gamma-Carboxylation | no | yes | yes | yes | |
Growth medium | minimum | minimum | complex | complex | |
Yield | >50% | 10% | >30% | <1% | |
Speed | fast | medium | medium | slow | |
Cost | low | low | medium | high | |
Scale up | **** | **** | ** | * |
YOUR CONCERNS
Will I get what I am promised?
Our counter-contract ensures your terms of expectation are met.
I feel like losing control of data generation!
Our service team keeps tight monitoring and tracking over each step where data are generated.