|Recombinant Human Left-right Determination Factor 1 (N-6His)|
|Description||Recombinant Human Left-right Determination Factor 1 is produced by our Mammalian expression system and the target gene encoding Phe78-Pro366 is expressed with a 6His tag at the N-terminus.|
|Names||Left-right determination factor 1; Left-right determination factor B; Protein lefty-1; Protein lefty-B; LEFTY1; LEFTB; LEFTYB|
|Formulation||Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.|
|Shipping||The product is shipped at ambient temperature.|
|Reconstitution||Always centrifuge tubes before opening. Do not mix by vortex or pipetting.|
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
|Storage||Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.|
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
|Purity||Greater than 95% as determined by reducing SDS-PAGE.|
|Endotoxin||Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.|
|Amino Acid Sequence|
LTGEQILGSLLQQLQLDQPPVLDKADVEGMVIPSHVRTQYVALLQHSHASRSRGKRHHHHHHFSQ SFREVAGRFLALEASTHLLVFGMEQRLPPNSELVQAVLRLFQEPVPKAALHRHGRLSPRSARARV TVEWLRVRDDGSNRTSLIDSRLVSVHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGP LASGAHKLVRFASQGAPAGLGEPQLELHTLDLGDYGAQGDCDPEAPMTEGTRCCRQEMYIDLQGM KWAENWVLEPPGFLAYECVGTCRQPPEALAFKWPFLGPRQCIASETDSLPMIVSIKEGGRTRPQV VSLPNMRVQKCSCASDGALVPRRLQP
|Background||Lefty was first identified in a screen for undifferentiated cellspecific cDNAs from the P19 mouse embryonal carcinoma cells. Its mRNA expression on the left side of the developing embryo earned it the name “l e f t y”. Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A(ebaf) and Lefty-B). Lefty contains the six cysteine residues that are conserved among TGF-β related proteins and that are necessary to form the cysteine-knot structure. Lefty homologues have been identified in other vertebrate organisms including chick, frog, and zebrafish. Although the amino acid sequence identity is not well conserved among vertebrate species, the expression pattern of lefty on the left side is well conserved. Lefty-1 is expressed strongly on the left side of the prospective floor plate and weakly in the left half of the lateral plate mesoderm in E8 mice embryos. In all species examined, lefty proteins function in patterning leftright asymmetry of the developing organ systems such as the heart and lung. Lefty acts as an antagonist to Nodal signaling, potentially by competing for binding to a common receptor.|
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