Custom Rabbit Polyclonal Antibody Service
Advantages of our polyclonal antibody production system: low cost, high titer, high purity, and high yield.
- Free Antigen Design: our in house experts possess extensive experience in designing optimal antigens based on your applications and protein characteristics.
- Flexible Antigen Choices: we generate antigens as peptides or recombinant proteins based on your budget and protein properties.
- 10 WeekImmunization Protocol: we immunize two rabbits in parallel for 10 weeks with a boost every two weeks for the best antibody production.
- Guaranteed ELISA Titer Result: we only charge our customers when the antibody has greater than 1:8000 ELISA titer.
- Deliverable: minimal delivery is 10mg with greater than 95% purity.
Antigen Preparation for Peptides
- Peptide: $20 per amino acid
- Antigen design and peptide synthesis: Free
- Conjugation of 5mg peptide to Keyhole Limpet Hemocyanin (KLH): $150
Antigen Preparation for Recombinant Proteins
- Protein analysis and antigen selection: free
- Gene synthesis and codon optimization: $0.40 per base pair
- Subclone gene of interest into E. coli expression vector: $195
- Expression optimization: $100
- Recombinant protein antigen production at 5mg: $600
- $600 to immunize 2 rabbits
- Boost 5 times over 10 weeks
- Periodic testing until anti-sera titer is at 1:8000
- Harvest of 100ml pooled sera
- Conjugate 2mg peptide antigen to NHS-activated Sepharose 4B or protein A bead purification
- Perform affinity chromatography to purify antibody
- Perform post-purification modifications as required