Protein expression with the mammalian cell expression system has the most comprehensive post-translational modifications with guaranteed activity.
Uniform isotope labeling is a key tool for NMR studies on recombinant proteins and their interaction with ligands of pharmaceutical interest. Most recombinant proteins in labeled form expressed in E. coli system are required to grow in minimal medium.
Structural information is one of the key steps to fully understand a proteins biological function. The goal of crystallization is to produce a well-ordered crystal in order to discern the proteins tertiary structure.more