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Service Highlights:

• High Efficiency: our collection of more than 10 host strains and various proprietary or non-proprietary vectors guarantees the selection of the most efficient expression system.
• High Productivity: multiple fusion tags and our proprietary AIE technology are available in house to increase protein solubility and production, and to decrease purification cost, even for toxic proteins.
• Refolding: we have established an effective protein refolding system consisting of 20 refolding buffers optimized with 9 parameters to increase the chance to obtain soluble and bioactive proteins.
• Large-Scale Production: we are able to produce up to 500L of E. coli cell culture for gram scale production.
• Modifications: metabolic labeling with stable isotopes (¹³C and ¹⁵N) and biotinylation are available.
• Competitive Pricing: 5mg protein at80% purity from only $1000!

Our Services:

Cloning and Expression

• Codon optimization and gene synthesis (optional): $0.40 per base pair
• Subcloning gene of interest into E. coli expression vector: $195 each
• Construction of expression strains and optimization of expression conditions: $100

Pilot Scale Expression and Production

• 2 liters of bacterial cell culture
• Inducing cells with IPTG and cell harvesting for purification
• Purifying protein with or without tag by affinity column, gel filtration, ion exchange, orhydrophobic column
• Removal of fusion/purification tag
• 5mg of protein from $1000

Large-Scale Production

• Large-scale fermentation up to 500L with 60-80g cell pellet/liter
• Large-scale purification up to gram scale

Additional Services

• Protein refolding
• Metabolic labeling with stable isotopes (¹³C and ¹⁵N)
• Biotinylation
• Large-scale fermentation
• Large-scale purification
• Process development