Recombinant Klebsiella pneumoniae Kanamycin kinase type II/NEO

Cat.No.: CM66

Recombinant K. pneumoniae NEO
Description
Recombinant Klebsiella pneumoniae Aminoglycoside 3'-phosphotransferase is produced by our E.coli expression system and the target gene encoding Met1-Phe264 is expressed.
Accession #:P00552
Known as:Aminoglycoside 3'-phosphotransferase; APH(3')-II; APH(3')II; Kanamycin kinase type II; Neomycin-kanamycin phosphotransferase type II; neo
Formulation
Supplied as a 0.2 μm filtered solution of PBS, pH 7.4, 20% Glycerol.
Quality Control
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Storage
Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.
Background
Aminoglycoside 3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3') is primarily found in certain species of gram-positive bacteria.

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