| Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1/UCH-L1 | |
| Catalog# | C188 |
| Source | E. coli |
| Description | Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1/UCH-L1 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ala223) of Human UCH-L1. |
| Names | Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1, UCH-L1, Neuron Cytoplasmic Protein 9.5, PGP 9.5, PGP9.5, Ubiquitin Thioesterase L1, UCHL1 |
| Accession # | P09936 |
| Formulation | Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 250mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5 |
| Shipping | The product is shipped on dry ice/ice packs. |
| Storage | Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles. |
| Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/μg (1 IEU/μg). |
| Amino Acid Sequence | MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKK QIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRA KCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLK DAAKVCREFTEREQGEVRFSAVALCKAALEHHHHHH |
| Background | Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 (UCHL1) belongs to the Peptidase C12 family. UCHL1 is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. UCHL1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. |
| MSDS |  |