Recombinant Human pro-Neurotrophin-3/pro-NT3
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10ug/$105

50ug/$315

500ug/$1540

1mg/$2200

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Catalog#C080
SourceE. coli
DescriptionRecombinant Human precursor form of Neurotrophin-3/pro-NT3 is produced by our E. coli expression system.
NamesNeurotrophin-3, NT-3, HDNF, Nerve Growth Factor 2, NGF-2, Neurotrophic Factor, NTF3
Accession #P20783
FormulationLyophilized from a 0.2 μM filtered solution of 20mM PB, 250mM NaCl, pH 7.2
ShippingThe product is shipped at ambient temperature.
ReconstitutionAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
StorageLyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
PurityGreater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
EndotoxinLess than 0.1 ng/μg (1 IEU/μg).
Amino Acid Sequence
MNNMDQRSLPEDSLNSLIIKLIQADILKNKLSKQMVDVKENYQSTLPKAEAPREPERGGPAKSAF QPVIAMDTELLRQQRRYNSPRVLLSDSTPLEPPPLYLMEDYVGSPVVANRTSRRKRYAEHKSHRG EYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKH WNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT
BackgroundPro-Neurotrophin-3 (pro-NT3) is the precursor form of Neurotrophin-3. Pro-NT3 interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature NT3 selectively binds with high affinity to the TrkB kinase receptor and promotes the survival, growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects, Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner.
ReferencesHoward L, et al. ProNGF promotes neurite growth from a subset of NGF-dependent neurons by a p75NTR-dependent mechanism
PMID: 23633509, PMCID: PMC3640218