|Recombinant Human pro-Brain-Derived Neurotrophic Factor/pro-BDNF|
|Description||Recombinant Human precursor form of Brain-Derived Neurotrophic Factor/pro-BDNF produced in E. coli is a non-glycosylated polypeptide chain containing 2 X 229 amino acids as a homodimer with a molecular mass of 52 kD.|
|Names||Brain-Derived Neurotrophic Factor, BDNF, Abrineurin|
|Formulation||Lyophilized from a 0.2 μM filtered solution of 20mM PB, 250mM NaCl, pH 7.2|
|Shipping||The product is shipped at ambient temperature.|
|Reconstitution||Always centrifuge tubes before opening. Do not mix by vortex or pipetting.|
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
|Storage||Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.|
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
|Purity||Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.|
|Endotoxin||Less than 0.1 ng/μg (1 IEU/μg).|
|Amino Acid Sequence|
MAPMKEANIRGQGGLAYPGVRTHGTLESVNGPKAGSRGLTSLADTFEHVIEELLDEDQKVRPNEE NNKDADLYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEW VTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQ SYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR
|Background||The precursor form of Brain-Derived Neurotrophic Factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival, growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects, Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.|