|Recombinant Human Interleukin-10/IL-10|
|Description||Recombinant Human Interleukin-10/IL-10 produced in E. coli is a single non-glycosylated polypeptide chain containing 160 amino acids with a molecular mass of 18,648 Daltons.|
|Names||Interleukin-10, IL-10, Cytokine Synthesis Inhibitory Factor, CSIF, IL10|
|Formulation||Lyophilized from a 0.2 μM filtered solution of 20mM PB, 300mM NaCl, pH 7.3|
|Shipping||The product is shipped at ambient temperature.|
|Reconstitution||Always centrifuge tubes before opening. Do not mix by vortex or pipetting.|
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
|Storage||Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.|
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
|Biological Activity||ED50 is less than 2 ng/ml as determined by the dose-dependent co-stimulation with murine IL-4 of MC-9 cells.|
Specific Activity of 1.5 x 106 IU/mg.
|Purity||Greater than 95% as determined by RP-HPLC and reducing SDS-PAGE.|
|Endotoxin||Less than 0.1 ng/μg (1 IEU/μg).|
|Amino Acid Sequence|
MSPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQA LSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFN KLQEKGIYKAMSEFDIFINYIEAYMTMKIRN