|Recombinant Human Interferon-α2b/IFN-α2b|
|Description||Recombinant Human Interferon-α2b produced in E. coli is a single non-glycosylated polypeptide chain containing 166 amino acids with a molecular mass of 19,400 Daltons. The Interferon-α2b gene was obtained from human leukocytes.|
|Names||Interferon Alpha-2, IFN-Alpha-2, Interferon Alpha-A, LeIF A, IFNA2|
|Formulation||Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2|
|Shipping||The product is shipped at ambient temperature.|
|Reconstitution||Always centrifuge tubes before opening. Do not mix by vortex or pipetting.|
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
|Storage||Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.|
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
|Biological Activity||Specific Activity is greater than 1.0 x 108 IU/ mg as determined by a viral resistance assay using VSV-WISH cells.|
|Purity||Greater than 95% as determined by RP-HPLC and reducing SDS-PAGE.|
|Endotoxin||Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.|
|Amino Acid Sequence|
MCDLPQTHSLGSRRTLMLLAQMRRISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIF NLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLY LKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE
|Background||At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end.|