| Recombinant Human Interferon-α2b/IFN-α2b | |
| Catalog# | C005 |
| Source | E. coli |
| Description | Recombinant Human Interferon-α2b produced in E. coli is a single non-glycosylated polypeptide chain containing 166 amino acids with a molecular mass of 19,400 Daltons. The Interferon-α2b gene was obtained from human leukocytes. |
| Names | Interferon Alpha-2, IFN-Alpha-2, Interferon Alpha-A, LeIF A, IFNA2 |
| Accession # | P01563 |
| Formulation | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2 |
| Shipping | The product is shipped at ambient temperature. |
| Reconstitution | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Biological Activity | Specific Activity is greater than 1.0 x 108 IU/ mg as determined by a viral resistance assay using VSV-WISH cells. |
| Purity | Greater than 95% as determined by RP-HPLC and reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Amino Acid Sequence | MCDLPQTHSLGSRRTLMLLAQMRRISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIF NLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLY LKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE |
| Background | At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end. |
| MSDS |  |