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10ug50ug500ug1mg
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Recombinant Human Deoxyribonuclease-1/DNase I
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novoprotein
Catalog#C599
SourceHEK293
DescriptionRecombinant Human Deoxyribonuclease-1/DNase I is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Leu23-Lys282) of Human DNASE1 fused with a polyhistidine tag at the C-terminus.
NamesDeoxyribonuclease-1, Deoxyribonuclease I, DNase I, Dornase Alfa, DNASE1, DNL1, DRNI
Accession #P24855
FormulationSupplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5
ShippingThe product is shipped on dry ice/ice packs.
StorageStore at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.
PurityGreater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
EndotoxinLess than 0.1 ng/μg (1 IEU/μg).
Amino Acid Sequence
LKIAAFNIQTFGETKMSNATLVSYIVQILSRYDIALVQEVRDSHLTAVGKLLDNLNQDAPDTYHY VVSEPLGRNSYKERYLFVYRPDQVSAVDSYYYDDGCEPCGNDTFNREPAIVRFFSRFTEVREFAI VPLHAAPGDAVAEIDALYDVYLDVQEKWGLEDVMLMGDFNAGCSYVRPSQWSSIRLWTSPTFQWL IPDSADTTATPTHCAYDRIVVAGMLLRGAVVPDSALPFNFQAAYGLSDQLAQAISDHYPVEVMLK HHHHHH
BackgroundDeoxyribonuclease-1 (DNase I) is an endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It belongs to the DNase I family and acts on single-stranded DNA, double-stranded DNA, and chromatin. It has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis. DNase 1 has also been a very useful tool in the isolation of actin from various sources by affiinity chomatography. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.

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