|Recombinant Human Bone Morphogenetic Protein 2/BMP-2|
|Description||Recombinant Human Bone Morphogenetic Protein 2/BMP-2 is produced by our E. coli expression system. The target protein is expressed with sequence (Gln283-Arg396) of Human BMP-2.|
|Names||Bone Morphogenetic Protein 2, BMP-2, Bone Morphogenetic Protein 2A, BMP-2A, BMP2, BMP2A|
|Formulation||Lyophilized from a 0.2 μM filtered solution of 50mM HAc|
|Shipping||The product is shipped at ambient temperature.|
|Reconstitution||Always centrifuge tubes before opening. Do not mix by vortex or pipetting.|
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 50mM Acetic Acid.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
|Storage||Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.|
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
|Biological Activity||Recombinant BMP-2 is fully biologically active when compared to standards.|
ED50 is less than 50 ng/ml as determined by the cytolysis of MC3T3-E1 cells.
Specific Activity of 2.0 x 104 IU/mg
|Purity||Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.|
|Endotoxin||Less than 0.1 ng/µg (1 IEU/µg).|
|Amino Acid Sequence|
|Background||Bone Morphogenetic Protein-2 (BMP-2) is one of the bone-growth regulatory factors that belong to the transforming growth factor-beta (TGF-beta) superfamily of proteins. BMPs are synthesized as large precursor molecules, which are cleaved by proteolytic enzymes. The active form of BMP-2 can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.|
|References||Wu T, et al. miR-30 family members negatively regulate osteoblast differentiation. PMID: 22253433, PMCID: PMC3293535 http://www.ncbi.nlm.nih.gov/pubmed/22253433|