| Recombinant Human Bone Morphogenetic Protein 2/BMP-2 | |
| Catalog# | C012 |
| Source | E. coli |
| Description | Recombinant Human Bone Morphogenetic Protein 2/BMP-2 is produced by our E. coli expression system. The target protein is expressed with sequence (Gln283-Arg396) of Human BMP-2. |
| Names | Bone Morphogenetic Protein 2, BMP-2, Bone Morphogenetic Protein 2A, BMP-2A, BMP2, BMP2A |
| Accession # | P12643 |
| Formulation | Lyophilized from a 0.2 μM filtered solution of 50mM HAc |
| Shipping | The product is shipped at ambient temperature. |
| Reconstitution | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Biological Activity | Recombinant BMP-2 is fully biologically active when compared to standards. ED50 is less than 50 ng/ml as determined by the cytolysis of MC3T3-E1 cells. Specific Activity of 2.0 x 104 IU/mg |
| Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/μg (1 IEU/μg). |
| Amino Acid Sequence | MQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQ TLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR |
| Background | Bone Morphogenetic Protein-2 (BMP-2) is one of the bone-growth regulatory factors that belong to the transforming growth factor-beta (TGF-beta) superfamily of proteins. BMPs are synthesized as large precursor molecules, which are cleaved by proteolytic enzymes. The active form of BMP-2 can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. |
| MSDS |  |