| Recombinant Human Uroporphyrinogen-III Synthase/UROIIIS | |
| Catalog# | C268 |
| Source | E. coli |
| Description | Recombinant Human Uroporphyrinogen-III Synthase/UROIIIS is produced by our E.coli expression system. The target protein is expressed with sequence (Met1-Cys265) of Human UROS fused with a 6His tag at the C-terminus. |
| Names | Uroporphyrinogen-III Synthase, UROIIIS, UROS, Hydroxymethylbilane Hydrolyase [Cyclizing], Uroporphyrinogen-III Cosynthase, UROS |
| Accession # | P10746 |
| Formulation | Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, 10% Glycerol, pH 8.0 |
| Shipping | The product is shipped on dry ice/ice packs. |
| Storage | Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles. |
| Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/μg (1 IEU/μg). |
| Amino Acid Sequence | MKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLIFTSPR AVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCGNAEKLAEYIC SRESSALPLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNLNSYYSQQGVPASITF FSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVSCTAESPTPQALATGIRKALQ PHGCCLEHHHHHH |
| Background | Uroporphyrinogen-III Synthase is an enzyme which belongs to the uroporphyrinogen-III synthase family. Uroporphyrinogen-III Synthase is ubiquitous and it is involved in Porphyrin metabolism. Porphyrins act as cofactors for a multitude of enzymes that perform a variety of processes within the cell such as Methionine synthesis (Vitamin B12) or oxygen transport (Heme). Uroporphyrinogen-III Synthase can catalyze cyclization of the linear Tetrapyrrole, Hydroxymethylbilane, to the Macrocyclic Uroporphyrinogen III, the branch point for the various sub-pathways leading to the wide diversity of Porphyrins. Defects in Uroporphyrinogen-III Synthase are the cause of Congenital Erythropoietic Porphyria (CEP). |
| MSDS |  |