|Recombinant Human N-Acetyllactosaminide β-1,3-N-Acetylglucosaminyltransferase/B3GNT1|
|Description||Recombinant Human N-Acetyllactosaminide β-1,3-N-Acetylglucosaminyltransferase/B3GNT1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Asp43-Cys415) of Human B3GNT1 fused with a polyhistidine tag at the C-terminus.|
|Names||N-Acetyllactosaminide Beta-1,3-N-Acetylglucosaminyltransferase, I-Beta-1,3-N-Acetylglucosaminyltransferase, iGnT, Poly-N-Acetyllactosamine Extension Enzyme, UDP-GlcNAc:BetaGal Beta-1,3-N-Acetylglucosaminyltransferase 1, B3GNT1, B3GNT6|
|Formulation||Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4|
|Shipping||The product is shipped at ambient temperature.|
|Reconstitution||Always centrifuge tubes before opening. Do not mix by vortex or pipetting.|
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
|Storage||Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.|
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
|Purity||Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.|
|Endotoxin||Less than 0.1 ng/µg (1 IEU/µg).|
|Amino Acid Sequence|
DQYFEFFPPSPRSVDQVKAQLRTALASGGVLDASGDYRVYRGLLKTTMDPNDVILATHASVDNLL HLSGLLERWEGPLSVSVFAATKEEAQLATVLAYALSSHCPDMRARVAMHLVCPSRYEAAVPDPRE PGEFALLRSCQEVFDKLARVAQPGINYALGTNVSYPNNLLRNLAREGANYALVIDVDMVPSEGLW RGLREMLDQSNQWGGTALVVPAFEIRRARRMPMNKNELVQLYQVGEVRPFYYGLCTPCQAPTNYS RWVNLPEESLLRPAYVVPWQDPWEPFYVAGGKVPTFDERFRQYGFNRISQACELHVAGFDFEVLN EGFLVHKGFKEALKFHPQKEAENQHNKILYRQFKQELKAKYPNSPRRCVDHHHHHH
|Background||N-Acetyllactosaminide β-1,3-N-Acetylglucosaminyltransferase (B3GNT1) is a member of the β-1,3-N-Acetylglucosaminyltransferase family. B3GNT1 is a single-pass type II membrane protein and widely expressed in many tissues. B3GNT1 can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans. B3GNT1 is essential for the synthesis of poly-N-acetyllactosamine, a determinant for the blood group i antigen. It can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans.|