Recombinant Human Transforming Growth Factor Beta-1/TGFB1
Cat.No.: CA59
Description
Recombinant Human Transforming Growth Factor beta 1 is produced by our Mammalian expression system and the target gene encoding Ala279-Ser390 is expressed.
Accession #:P01137
Known as:Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated Peptide; LAP; TGFB1; TGFB
Formulation
Lyophilized from a 0.2 μm filtered solution of 50mM Glycine-HCl, 150mM NaCl, pH2.5.
Quality Control
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:Less than 0.01 EU/µg as determined by LAL test.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Background
Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone, articular cartilage and chondrocytes and is increased in osteoarthritis (OA). TGFβ-1 performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide. TGFβ-1 may also form heterodimers with other TGFβ family members. It has been found that TGFβ-1 is frequently upregulated in tumor cells. Mutations in this gene results in Camurati-Engelmann disease.
Publication
KrasG12D mutation contributes to regulatory T cell conversion through activation of the MEK/ERK pathway in pancreatic cancer He Cheng, et al. (Cancer Letters, 2019)+
Immortalized Hertwig's epithelial root sheath cell line works as model for epithelial–mesenchymal interaction during tooth root Sicheng Zhang, et al. (journal of cellular physiology, 2019)+
Neuropilin 1 modulates TGFβ1induced epithelialmesenchymal transition in nonsmall cell lung cancer ZONGLI DING, et al. (International Journal of Oncology, 2019)+
The role and molecular mechanism of Trop2 induced epithelial‐mesenchymal transition through mediated β‐catenin in gastric cancer Wei Zhao, et al. (Cancer Medicine, 2019)+
Andrographolide ameliorates bleomycin-induced pulmonary brosis by suppressing cell proliferation and myofibroblast differentiat Jingpei Li, et al. (Toxicology Letters, 2019)+
Sialylation of vasorin by ST3Gal1 facilitates TGF‐β1 mediated tumor angiogenesis and progression Hui Ling Yeo , et al. (INTERNATIONAL JOURNAL OF CANCER, 2018)+
TGF-β1 resulting in differential microRNA expression in bovine granulosa cells YefenXu, et al. (Gene, 2018)+
P16 INK4a Deletion Ameliorated Renal Tubulointerstitial Injury in a Stress-induced Premature Senescence Model of Bmi-1 Deficienc Jianliang Jin, et al. (Scientific Reports, 2017)+
Human mesenchymal stem cells cultured on silk hydrogels with variable stiffness and growth factor differentiate into mature smoo Floren M, et al. (Acta biomaterialia, 2016)+
High-efficiency reprogramming of fibroblasts into cardiomyocytes requires suppression of pro-fibrotic signalling Zhao Y, et al. (Nature communications, 2015)+
